Abstract

Title : PROTECTION OF MELANOCYTE DAMAGE AND MELANOGENESIS STIMULATING ACTIVITY OF THAI TRADITIONAL PLANT EXTRACTS USING FOR GRAY HAIR
By : KITTIYARAT SOMVONG
Degree : MASTER OF SCIENCE
Major : PHARMACEUTICAL CHEMISTRY AND NATURAL PRODUCTS
Advisor : CHUTINUN PRASITPURIPRECHA
Keywords : MELANOGENESIS / MOUSE MELANOMA CELL LINES (B16F10) /THAI TRADITIONAL MEDICINAL PLANT / GRAY HAIR
   
The objective of this research was to investigated the melanogenesis stimulating activity of five Thai traditional plant extracts such as aqueous, ethyl acetate, methanol and hexane extracts of Clitoria ternatea L., Tiliacora triandra (Colebr.) Diels., Centella asiatica (L.) Urb., Morus alba L. and Pueraria candollei Graham ex Benth. var mirifica (Airy Shaw et Suvat.) Niyomdham. Those extracts were investigated for antioxidant activity, protective effect of melanocyte from free radical of hydrogen peroxide, stimulating activity of melanocyte proliferation, stimulating activity of tyrosinase enzyme and stimulating activity of melanin synthesis. The results of antioxidant activity by DPPH assay, ABTS assay and FRAP assay showed that methanol and aqueous extract of T. triandra had strong activity with the EC50, VEAC, TEAC and FRAP value of 61.8 µg/ml, 2.00 mM, 0.77 mM and 546.9 mM, respectively. It also had the highest quantity of total phenolic compounds with 82.75 µg GAE/ mg dry mass. The results of protective effect of melanocyte from free radical of 2 mM hydrogen peroxide by MTT assay exhibited that aqueous extracts of C. asiatica, T. triandra and C. ternatea had good activities with cell viability of 89.62%, 83.14% and 80.62%, respectively. The results of stimulating activity of melanocyte proliferation by MTT assay showed that methanol extracts of C. ternatea, aqueous extract of T. triandra and hexane extract of C. asiatica had strong activities with proliferation index (P.I.) of 1.73 and 1.59 and 1.25, respectively. The results of stimulating activity of tyrosinase enzyme by tyrosinase activity assay showed that ethyl acetate extracts of T. triandra and C. asiatica and hexane extract of C. ternatea had strong activities with stimulation index of 12.78, 12.72 and 9.92, respectively while the results of this activity in cell culture through DOPA oxidase activity assay showed that methanol extracts of C. ternatea and C. asiatica and ethyl acetate of T. triandra had strong activities with % of control of 157.97, 147.01 and 144.61, respectively. The results of stimulating activity of melanin synthesis by measuring melanin content exhibited that aqueous extract of C. asiatica and T. triandra and ethyl acetate extract of C. ternatea had strong activities with % of control 231.1, 183.3 and 166.66, respectively. The chemical identification and TLC fingerprints of these extracts were also done. Almost extracts composed of alkaloids, anthraquinones, anthrones, coumarines, cardiac glycosides, flavonoid, carotenoid, tannin, xanthone and cardenolides. This can be concluded that the potential medicinal plants having melanogenesis stimulating activities were aqueous, ethyl acetate, methanol and hexane extracts of T. triandra, C. ternatea and C. asiatica. These can be able to developed for anti-gray hair cosmetic in the future.
   
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