Abstract

PRELIMINARY SCREENING TEST FOR THE EFFECT OH HOLY BASILAND 4 HERBAL EXTRACT ON RAT CYP2D6 ENZYME ACTIVITY IN-VITRO

DUANGJAI JANPIRAK SASITORN LEEPRAKON

BENJABHON SETHABOUPPHA SUTTASINEE SUWANNAKUL

CYP450, CYP2D6, DEXTROMETHORPHAN, DEXTRORPHAN

The objective of this study are priliminary screening test the effect of Ocimum sanctum and other 4 Thai herbal crude extracts on function of rat CYP2D6 enzyme. Metabolic tests were carried out in duplicate in vitro using Dextromethorphan as a specific CYP2D6 prove substrate. The amount of Dexthorphan, major metabolite of Dextromethorphan, obtained from each metabolic reaction with or without herbal crude extracted (in alcohol and water) was determined by using High Performance Liquid Chromatography (HPLC) technique then converted into % inhibition. Result revealed that the aqueous extract of 4 herbs was affected CYP2D6 enzyme function in different degree of inhibition i.e. holy basil (Ocimum sanctum L.) 54.29 %, ivy gourd (Coccinia grandis) 15.84 %, Indian mulberry leaf (Morinda citrifolia) 30.30 % and bamboo grass (Tiliacora triandra) 3.42 %. The alcoholic extracts of 5 herbs inhibited CYP2D6 activity i.e. holy basil (Ocimum sanctum L.) 4.86 %, ivy gourd 11.74 %, Indian mulberry leaf 65.65 %, star gooseberry (Sauropus androgynus) 22.46 % and bamboo grass 5.48 %. It could be conclude that all 5 Thai herbs inhibit CYP2D6 function in different degree.