| Acinetobacter spp. is a gram negative bacterium causing the high incidence of multi-drug resistance in patients admitted to an intensive care unit. Objectives= The objectives of this study were to detect the productions of carbapenemases and antibiotic resistant genes of carbapenem-resistant Acinetobacter spp. at Sunpasitthiprasong Hospital. Materials and Methods= One hundred isolates of imipenem-resistant Acinetobacter spp. isolated from patients admitted at Sunpasitthiprasong Hospital were used in this study. Productions of carbapenemases were tested by modified Hodge test and combined disc test. Antibiotic resistant genes, blaOXA-like, involving in production of Class D carbapenemases were detected by multiplex polymerase chain reaction. Results= The detection of carbapenemases production among 100 isolates revealed that 35 and 33 isolates were found to be carbapenemases producer by the modified Hodge test and combined disc test, respectively. For the detection of blaOXA-like genes, 92, 92, and 8 isolates were detected for blaOXA-51-like, blaOXA-23-like and blaOXA-58-like genes, respectively. No detection of blaOXA-24-like gene was observed. Conclusion= Carbepenemases production may be an important mechanism of carbapenem resistance among clinical isolates of Acinetobacter spp. at Sunpasitthiprasong Hospital. The most common types of class D carbapenemases genes contributing to carbapenem resistance in these isolates are blaOXA-51-like and blaOXA-23-like. |