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Abstract |
| Title |
: PRELIMINARYSCREENING TEST FOR THE EFFECT OFPHAK-WAN AND 3 THAI HERBAL EXTRACTS ON RAT CYP3A4 ENZYME ACTIVITY IN-VITRO |
| By |
: CHANITSARA BOONCHAROEN PITIKARN CHEABKOR SIRAPORN SITTHIRAM |
| Degree |
: DOCTOR OF PHARMACY |
| Advisor |
: BENJABHON SETHABOUPPHA SUTTASINEE SUWANNAKUL |
| Keywords |
: Herb-drug interaction, Cytochrome P450, CYP3A4, Tiliacoratriandra, Ocimumsanctum,Cocciniagrandis, Tiliacoratriandra |
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| In the present, people are widely used Thai herbal medicine for treatment of common illness ether as a single drug or combination with other modern drugs. It is possible to cause herbal drug interaction by affecting to Cytochrome P450 enzyme function. This research aimed to study the effect of Tiliacoratriandra and other 3 Thai herbalcrude extracts on function of rat CYP 3A4 enzyme. Metabolic tests were carried out in duplicate in vitro using Testosterone (TST) as a specific CYP 3A4 probe substrate. The amount of 6β-hydroxytestosterone (6β-TST), major metabolite of TST, obtained from each metabolic reaction with or without herbal crude extracted (in alcohol) was determined using High Performance Liquid Chromatography (HPLC) technique then converted into % inhibition. Result reviewed that the alcohol extracts 4 herbs were affected CYP 3A4 enzyme function in different degree of inhibition i.e. Phak-wan (Sauropus androgynus (L.) Merr.)(7.56%), Kaprao (Ocimum sanctum L.)(57.91%), Tam-lueng (Coccinia grandis(L.)Voigt.)(0.95%) and Ya-nang (Tiliacora triandra Diels.)(1.05%). It could be conclude that all 4 Thai herbs inhibit CYP3A4 function in different degree. |
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