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Abstract |
| Title |
: SCREENING IN VITRO ANTIBACTERIAL PROPERTIES OF THAI HERBAL EXTRACTS AGAINST ACINETOBACTER BAUMANNII AND MULTI-DRUG RESISTANT STRAINS CAUSING NOSOCOMIAL INFECTION AT SUNPASITTHIPRASONG HOSPITAL |
| By |
: PARIYAPORN POSRI PHETCHARAT SAMEK |
| Degree |
: DOCTOR OF PHARMACY |
| Advisor |
: TEERAPONG MONMATURAPOJ SOMWANG JANYAKHANTIKUL |
| Keywords |
: ACINETORBACTER BAUMANNII, MULTI-DRUG RESISTANT ACINETORBACTER BAUMANNII, THAI HERBAL EXTRACTS, ANTIBACTERIAL ACTIVITY, AGAR WELL DIFFUSION MEDTHOD |
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| Acinetobacter baumannii (A. baumannii) is an important cause of nosocomial infection and related with high mortality rate. Currently, antibacterial resistant of this pathogen has been increasing. It may lead to clinical treatment failure. Herbals medicine may offer alternative treatment option. Objectives= This study was screening antibacterial activity of Thai herbal extracts for against A. baumannnii and multi-drug resistant A. baumannii (MDR- A. baumannii) and investigate the antimicrobial susceptibility at Sunpasitthiprasong hospital, Ubon Ratchathani. Method= From 60 samples of A. baumannii and MDR- A. baumannii isolated from blood, pus and sputum of patients were collected. There was selected 7 sample to test antibacterial activity (5 samples of MDR- A. baumannii and 2 samples of A. baumannii). All isolates were examined antibacterial activity with 10 herbal extracts by using agar well diffusion method. Each well was fill 130 µl at the concentration of 10 mg/ml of herbal extracts in 10 percent volume/volume dimethyl sulfoxide solvent . According to CLSI 2012, there were incubated at 37 ๐C for 20 - 24 hours to examine zone of inhibition. Result= The result demonstrated Caesalpinia sappan and Camellia sinensis were the most potent herbal extract which inhibitory zone ranging from 14-17 mm and 11-14 mm, respectively. The positive control in our study was colistin sulfate 0.07 µg/µl and demonstrated the inhibitory zone ranging from 13-17 mm. Antimicrobial susceptibility in our study showed that A. baumannii were highly susceptibility to sulbactam, imipenem nd meropenem. Whereas, MDR- A. baumannii had a high number of resistance rate (80-90 %). All selected samples were susceptible to colistin which a treatment of choice in the present. Conclusion= Caesalpinia sappan had an excellent antibacterial activity. It might help to identify novel antibacterial agent especially against highly resistance pathogens. |
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