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Abstract |
| Title |
: EVALUATION OF PROBIOTIC POTENTIAL OF LACTIC ACID BACTERIA ISOLATED FROM FERMENTED FISH |
| By |
: WARAWUT WIROPROM AND WEERAYA SRISURIYACHAI |
| Degree |
: DOCTOR OF PHARMACY |
| Advisor |
: ASSIST. PROF. SIRIMA SUVARNAKUTA JANTAMA, Ph.D., AND ASSIST. PROF. KAEMWICH JANTAMA, Ph.D. |
| Keywords |
: PROBIOTIC, LACTIC ACID BACTERIA, FERMENTED FISH |
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| Probiotics are viable microorganisms that exhibit a beneficial effect on the health of human being by improving the intestinal microbial balance. Objective= The aim of this study was to evaluate the potential probiotic properties of lactic acid bacteria isolated from fermented fish. Methods= Fermented fish (Pla-Som) were collected from local markets for isolation of lactic acid bacteria. Preliminary identification of lactic acid bacteria were tested by gram staining and catalase test. Antimicrobial activities against 4 pathogenic bacteria were screened by agar diffusion using cylinder cup. In order to evaluate the tolerance in gastrointestinal conditions, the bacterial isolates of lactic acid bacteria showing the antagonistic activity against all four pathogenic bacteria were selected for further analysis of the acid (pH 2 and pH 3) and bile salt tolerance. Antimicrobial susceptibility of the selected strains was also investigated by agar disc diffusion. Results= According to the preliminary identification, a total number of 40 isolates of gram-positive bacteria with catalase negative were selected for further evaluation for probiotic properties. The results of antimicrobial test showed that 9 isolates of lactic acid bacteria could inhibit all tested pathogens. The bacterial isolate, FW1L-03 showed the highest survival rate at the acid condition of pH 2. All of 9 isolates were able to survive at pH 3 and bile salt after the incubation for 3 h. These selected isolates were sensitive to ampicillin, tetracycline, gentamicin and imipinem, but resistant to norfloxacin and sulfamethoxazole. Conclusion= Our findings suggest that the isolated lactic acid bacteria could be considered as antibacterial probiotics. However, further study is required to examine the other probiotic properties of these strains. |
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