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Abstract |
| Title |
: ANALYSIS OF CHEMICAL COMPOSITIONS OF CINNAMOMUM STOMACHIC MIXTURE FROM TRAKANPUETPON HOSPITAL= A GUIDE TO QUALITY CONTROL |
| By |
: CHANATHIP KWANKONG AND PATTARAPONG SIRISATEAN |
| Degree |
: DOCTOR OF PHARMACY |
| Advisor |
: KUSUMA JITSAENG, Ph.D AND RAWIWAN KAEWAUMMATAWONG,Ph.D |
| Keywords |
: CINNAMOMUM STOMACHIC, QUALITYCONTROL, THIN LAYER CHROMATOGRAPHY, CHEMICAL FINGERPRINT |
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| Cinnamomum stomachic mixture is a poly-herbal medicine that is highly prescribed for flatulence in Trakanpuetpon hospital, Ubon Ratchathani. The medicinal preparation was used and produced based on traditional practice however the standard method in quality control of the product has not been established. Objectives= To analyze the chemical components of Cinnamomum stomachic mixture and create chemical fingerprint for quality control as well as determination of antimicrobial activity of the medicine. Methods= In house formulations from different sources of medicinal plants were prepared. The mixture was freeze-dried and then extracted with MeOH. The chemical composition was analyzed by TLC. The TLC fingerprint was created by varying the developing solvent systems and methods of detection. The antimicrobial activity was evaluated by Disc Diffusion method. Results= The TLC analysis showed that the in house formulation and the hospital products contained eugenol, cinnamaldehyde, menthol and glycyrrhizhic acid. The TLC fingerprint of the extract was created by using two different solvent systems. They were a mixture of EtOAc and hexane (9=1) and a mixture of formic acid= glacial acetic acid= water (15=1=1=2) check system. This fingerprint was able to assess the quality control of the mixtures. The extracts of the mixtures (1 mg/disc) showed no antibacterial activity against the tested pathogens. Conclusions= The TLC methods were developed for analysis of chemical component in Cinnamon stomachic mixture. The chemical fingerprint was created and was able to used in preliminary quality control of Cinnamomum stomachic mixture from Trakanpuetpon hospital. |
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