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Abstract |
| Title |
: THE EFFECT OF KAM-PAENG-JED-CHAN AND 5 THAI HERBAL CRUDE EXTRACTS ON RAT CYP3A4 ENZYME ACTIVITY IN-VITRO |
| By |
: SAISUDA DEEWONG AND ORANEE PATTHATI |
| Degree |
: DOCTOR OF PHARMACY |
| Advisor |
: ASST.PROF.DR. BENJABHORN SETHABOUPPHA DR. SUTTASINEE SUWANNAKUL |
| Keywords |
: Herbal-drug interaction, Cytochrome P450, CYP3A4, Salacia chinensis L., Albizia myriophylla Benth., Ficus foveolata Wall., Solanum trilobatum L., Pandanus odoratissimus L.f. |
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| Herbal medicines are increasingly used for treatment of common illnesses either a single or combination with other modern medicines. Using some herbs with modern medicines may cause herbal-drug interaction by affecting to the functions of cytochrome P450 enzymes. Objectives= This research aimed to study the effect of Kam-Paeng-JedChan (Salacia chinensis L.), Cha-Em-Thai (Albizia myriophylla Benth.), Ma-Kra-Theub-Rong (Ficus foveolata Wall.), Ma-Waeng-Kreau (Solanum trilobatum L.) and Lum-Jeak (Pandanus odoratissimus L.f.) crude extracts on rat CYP3A4 enzyme activity in vitro. Methods= Metabolic test was testosterone 6β-hydroxylation that was carried out in triplicates in vitro by using testosterone (TST) as a specific CYP3A4 probe substrate. The amount of 6β- hydroxytestosterone, major TST metabolites, was determined by comparison between tube with and without herbal crude extract in High Performance Liquid Chromatography (HPLC) technique. Data was converted to percentage inhibitory activity of CYP3A4 enzyme. Results= The methanol extracts of 5 herbs inhibited CYP3A4 activity; Kam-Paeng-Jed-Chan 10.42%, Cha-Em-Thai 11.25%, Ma-Kra-Theub-Rong 34.79%, Ma-Waeng-Kreau 57.92% and Lum-Jeak 1.67%. The hexane extracts of 1 herb inhibited CYP3A4 activity; MaKra-Theub-Rong 2.46%. The aqueous extracts of 4 herbs inhibited CYP3A4 activity; Cha-Em-Thai 2.21%, MaKra-Theub-Rong 2.40%, Ma-Waeng-Kreau 5.54% and Lum-Jeak 0.25%. Conclusions= All 5 Thai herbal crude extracts have different degree of inhibitory effect on rat CYP3A4 enzyme activity in vitro. |
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