| The present study was aimed to investigate the antioxidative effect of Thai herbs used in traditional women remedies i.e. Carthamus tinctorius, Leonurus sibiricus, Caesalpinia sappan, Conioselinum univitatum, Curcuma aeruginosa, and Curculigo latifolia. The studies were composed of total phenolic content determination, free radical scavenging activity; DPPH and ABTS assay, and reducing power; FRAP and FTC assay compared with vitamin C, vitamin E, and trolox. The results showed that phenolic contents of Caesalpinia sappan and Curculigo latifolia were 671.407±8.455 and 152.222±1.626 mg/g,respectively. The free radical scavenging activities were examined by DPPH which demonstrated a scavenging power with IC50 of 7.31±0.099 and 38.44±0.728 µg/ml for Caesalpinia sappan and Curculigo latifolia, respectively. The results of ABTS assay expressed in TEAC (Trolox Equivalent Antioxidative Capacity) of Caesalpinia sappan and Curculigo latifolia were 415.831±9.984 and 119.067±4.616 µg/g, respectively. The reducing power of Caesalpinia sappan and Curculigo latifolia studied by using FRAP assay were 4817.374±89.386 and 154.747±33.804 µg of FeSO4 /mg respectively. The FTC assay demonstrated percent of antilipidperoxidation activity at the day7 showed that Leonurus sibiricus had the highest and higher than those of Conioselinum univitatum, Curculigo latifolia, Caesalpinia sappan, Carthamus tinctorius, and Curcuma aeruginosa, respectively.
|