| Keywords |
:
KEYWORDS : Herb-Drug
interaction, Cytochrome P450, CYP3A4, Nymphaea
lotus, Syzygium aromaticum,
Cinnamomum verum, Glycyrrhiza
glabra, Cinamomum bejolghota,
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ABSTRACT
TITLE :
EFFECT OF BUO-SAI CRUDE EXTRACTS AND OTHER 4 HERBS USED IN STOMACHIC MIXTURE ON CYP3A4 ACTIVITY
AUTHORS :
PONGRAT POKLIN AND PAWINEE AONIN
DEGREE :
DOCTOR OF PHARMACY
MAJOR :
PHARMACEUTICAL SCIENCES
CHAIRS : ASS.PROF.
DR. BENJABHORN SETHABOUPHA AND DR.SUTTHASINEE SUWANNAKUL
KEYWORDS : Herb-Drug
interaction, Cytochrome P450, CYP3A4, Nymphaea
lotus, Syzygium aromaticum,
Cinnamomum verum, Glycyrrhiza
glabra, Cinamomum bejolghota,
This research was aimed to study the effects of Buo-Sai crude extracts
and other 4 herbs used in stomachic mixture on CYP3A4 enzyme activity.
Metabolic tests was carried out in-vitro using rat liver microsome and
testosterone. The amount of 6β-Hydroxytestosterone, obtained from each
metabolic reaction with or without herbal crude extracts was determined by
using High Performance Liquid Chromatography (HPLC) technique then converted
into %inhibition. The results revealed that the aqueous extracts from 5 parts
of Buo-Sai (Nymphaea lotus) were
affected onto CYP3A4 enzyme activity in different degree of inhibition i.e. petal
30.16%, leaf 27.32%, flower stalk 17.52%, leaf stalk 6.3% and pollen 2.64%. The
aqueous extracts from 4 herbs of stomachic mixture i.e. Mixture of these 4
herbal extracts 26.07%, Sa-Mul-Wang (Cinamomum bejolghota) 22.8%, Cha-Em-Tes (Glycyrrhiza glabra) 17.25%, Ob-Choei-Tes (Cinnamomum verum) 16.77%,
Kan-Plu (Syzygium aromaticum) 4.28%, and mixture of these 4 herbal
extracts plus menthol and camphor 5.39%. The
hexane extracts from 5 parts of Bau-Sai (Nymphaea
lotus) i.e. petal 29.91%, leaf stalk 19.94%, pollen 16.34%, leaf 7.17% and flower stalk 2.88%. The alcohol extracts
from 5 parts of Bau-Sai (Nymphaea lotus)
i.e. pollen 50.42%, leaf stalk 35.59%, leaf
30.86%, petal 27.05%, flower stalk and 14.78%. It could be conclude
that the different part and different kind of herbs have different inhibitory effect
onto activity of CYP3A4 |