| Cratoxylumformosumsubsp. formosum(Cff, Clusiaceae), known in Thai as “TeawKhaw”, is a medicinal plant widely distributed in South East Asia. In traditional medicine, stems and leaves were used as anti-nausea and vomiting, anti-inflammatory and diuretic. Biological studies of Cffextracts and its secondary metabolites reported cytotoxic, antioxidant and antimicrobial activities. Objectives:The objectives of this study were to isolate endophytic fungi from Cff and screen for antioxidant and antimicrobial activities. Methods: The plant was collected from UbonRatchathani Province. The endophytic fungi were isolated by surface sterilization technique. The isolated fungi were cultured in potato dextrose broth. The culture media were partitioned with EtOAc while the hyphae were extracted with MeOH. The antioxidant activity of the extracts was evaluated by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging assay. Antioxidant activity was expressed in the half maximal inhibitory concentration (IC50, mean±S.D).Antimicrobial activity was tested by broth dilution methodand expressed in the percentage inhibition (%inhibition).Results: The isolates that showed strong antioxidant activity were Cff20, Cff08 and Cff17 with the IC50s of 13.7±4.5, 22.7±4.8 and 16.9±4.7 µg/ml, respectively. The isolates that showed strong antimicrobial activity wereCff12cell,Cff11cell and Cff04 with the percent inhibitions of 90.1±2.0, 90.00±1.7and 74.7±8.4, respectively. Conclusion: This study has illustrated that the isolated endophytic fungi exhibited the antioxidant and antimicrobial activities, which are the same as reported in host plant. These fungal endophytes could be used as alternative source of C. formosumsubsp. formosum. |