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Abstract |
| Title |
: Effect of Bastard cardamom (Amomum villosum Lour.) and other 2 Thai herbal crude extracts on rat CYP3A4 enzyme activity in-vitro |
| By |
: Naphatsorn Samranchai and Wisut Hirunpongchai |
| Degree |
: DOCTOR OF PHARMACY |
| Advisor |
: Dr.Suttasinee Suwannakul and Asst.Prof.Dr.Benjabhorn Sethabouppha |
| Keywords |
: Cytochrome P450, CYP3A4, Herb-Drug Interaction, Bastard cardamom (Amomum villosum Lour.), Shoebutton ardisia(Ardisia elliptica Thunb), Kaempfer (Boesenbergia rotunda (Linn.) Mansf.) |
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| The use of herbal supplements has increased and someherbal product can alter drug metabolism due to the interaction between herbs and medications through P450, major metabolizing enzyme. Objectives: This research aimed to study the effect of 3 Thai herbal crude extracts on function of rat CYP3A4 enzyme. Methods: Metabolic test were carried out in vitro using testosterone as a specific CYP3A4 probe substrate. The amount of 6β-hydroxy-testosterone obtained from metabolic reaction with or without herbal crude extract was determined using HPLC then converted into %inhibition. Results: Result revealed that the aqueous extract of 3 herbs inhibit CYP3A4 enzyme i.e. Bastard cardamom (Amomum villosum Lour.) 33.07%, Shoebutton ardisia(Ardisia elliptica Thunb)34.57% and Kaempfer (Boesenbergia rotunda (Linn.) Mansf.) 37.60%. The methanol extracts of 3 herbs inhibit CYP3A4 activity i.e. Bastard cardamom26.93%, Shoebutton ardisia30.86% and Kaempfer 52.03%. The hexane extract of 3 herbs also inhibit CYP3A4 activity i.e. Bastard cardamom67.80%, Shoebutton ardisia61.77% and Kaempfer 50.75%. Conclusions: It could be conclude that all 3 Thai herbs inhibit CPY3A4 function in different degree. |
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