Abstract

Title : The study ofrelationshipbetween KIR2DS3 and incidence of chronic hepatitis Cvirus infection
By : Chattriyaporn Muakthaisong and Pipatsorn Luekhajorn
Degree : DOCTOR OF PHARMACY
Advisor : Asst.Prof.Dr.Somwang Janyakhantikul
Keywords : Chronic hepatitis C virus, Thai population, KIR2DS3, PCR-SSP
   
Chronic hepatitis C virus infection presents a major problem for the public health in the world and Thailand. At the present, there is no available vaccine for protection the hepatitis C virus. The data from WHO 2014, 185 million people globally have chronic hepatitis C infection and 350,000 people die each year. In Thailand(2010), the prevalence of chronic hepatitis C infection is about150,000 people and patients who do not receive therapy may develop cirrhosis (20-35%) and/or hepatocellular carcinoma (3-6%).There are studies demonstrate that KIR2DS3have association with the susceptibility to hepatitis C virusinfection. However, there is no study to confirm this association in Thai population.Objectives:This study aims to investigate the relationshipbetween KIR2DS3 and incidence of chronic hepatitis C virus infection. Methods: Peripheral blood samples were collected from 100 chronic hepatitis C virus infection patients and 100 volunteers who are tested negative for hepatitis C virus infection. Then, the bloods were extracted for DNA by QIAamp DNA Blood mini kit, and the DNAs were examined for KIR2DS3 by Polymerase Chain Reaction-Sequence Specific Primer (PCR-SSP). Finally, the data of presence KIR2DS3found in both groups were compared for difference by Pearson’s Chi-squared Test. Results: The KIR2DS3 was found in 31 chronic hepatitis C virus infectionpatients (31%) and 22volunteers who are tested negative for hepatitis C virus infection(22%), respectively. However, there is no significant difference of the presence of KIR2DS3between these two groups (P-value=0.149).Conclusions:KIR2DS3is not related to the incidence of chronic hepatitis C virus infection in Thai population.
   
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