Abstract

Title : Optimization of extraction procedure for phenolic compounds in Piper sarmantosumRoxb.
By : Nida Nakharungsu and Passamon Nantapatavee
Degree : DOCTOR OF PHARMACY
Advisor : Dr.Kusuma Jitsaeng
Keywords : Piper sarmentosum,total phenolic contents (TPC), total flavonoid contents (TFC) antioxidant activity, optimization of extraction
   
Piper sarmentosumRoxb. (PS, Piperaceae) is a traditional herb used in treatment of many age-related diseases. Previous studies revealed that polyphenolic compounds were responsible for its bioactivities. Due to its availability and pharmacological properties, PS could be developed as health promoting products. Thus, the extract with high bioactive compounds is needed. Objectives: The purposes of this study were to study the effects of drying methods and solvents on total phenolic contents in PS extracts. Methods: Leaves of PS were dried in three different methods, oven drying (50C) air drying, and freeze drying, and then pulverized. Plant materials were extracted (1g/40ml) with mixture solvents of H2O and EtOH. They were H2O, 30%, 50%, 70% and 95% EtOH in H2O. Total phenolic (TPC) and flavonoid contents (TFC) as well as antioxidant activity (DPPH assay and ABTS assay) were determined to evaluate to quality of the extracts. Results: Drying conditions and solvents had affected the quality of the extracts. The 70% EtOH-extracts of air dried and oven dried materials contained the highest total phenolic contents and exhibited the highest antioxidant activity by DPPH assay. The 70% EtOH-extracts of oven dried material exhibited the highest antioxidant activity by ABTS assay. The 70% EtOH-extracts of air driedmaterial and 50% EtOH-extracts of oven dried material provided the highest total flavonoid contents. Conclusion: In this study, the optimum drying method and solvent to extract bioactive compound from PS were identified in stated condition.
   
Close this window