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Abstract |
| Title |
: Comparison of chemical constituents and biological activities between Commercial Calendula officinalis mother tinctures and homeopathic preparations of various marigolds grown in Thailand |
| By |
: Kanokporn Bunkanwanicha
Kittiyaporn Chairat
Methiya Phongam |
| Degree |
: BACHELOR OF PHARMACY |
| Advisor |
: Wandee Rungseevijitprapa
Chutinun Prasitpuriprecha
Chaiwat Chaisut
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| Keywords |
: Homeopathy, Antimicrobial, Analgesic, Calendula officinalis Linn, Tagetes erecta Linn.
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| The purpose of this study was to compare chemical constituents and biological activities between mother tinctures and homeopathic preparations of marigolds grown in Thailand. Mother tinctures and homeopathic dilutions were prepared from four marigolds; Calendula officinalis and Tagetes erecta (each species includes orange and yellow flowers). Chemical constituents as well as stability of the mother tinctures were assessed by Thin Layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC) using lutein as a phytochemical marker. Antimicrobial activity of mother tinctures and dilutions against 6 microorganisms; Staphylococcus aureus, Staphylococcus epidemidis, Bacillus cereus, Bacillus subtilis, Enterococcus faecalis and Pseudomonas aeruginosa were evaluated by agar diffusion method. Analgesic activity were tested in mice by Tail-flick test. Results showed that mother tincture of T. erecta was stable up to 40 days while Calendula officinalis lost its stability within 7 days. All dilutions exhibited no antimicrobial activity. However, homeopathic dilutions of Calendula officinalis (30 CH) help mice to tolerate pain better than those received Tramadol hydrochloride and better than the commercial Calendula officinalis while Tagetes erecta showed no activity. In conclusion, Calendula officinalis grown in Thailand has a potential to be developed as analgesic homeopathic remedy.
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