Abstract

Title : Study of Tyrosinase and DPPH inhibitor Activities from Artabotrys hexapetalus
By : Jumnean Somanawat Nusorn Thalaengsri Saijai Deponngam
Degree : BACHELOR OF PHARMACY
Advisor : Rawiwun Khawamtawong Nuttiya Werawattanachai
Keywords : Artabotrys hexapetalus, Annonaceae , Tyrosinase inhibition, DPPH
   
The objective of this study was to isolate and identify phytochemical groups that inhibit the melanin production and free radical scavenging activity from leaves of Artabotrys hexapetalus. From the results, ethyl acetate extract showed the tyrosinase inhibitory activity at %inhibition 24.37±13.62 and methanol showed DPPH radical scavenging activity at %inhibition 46.57±1.38. Ethyl acetate and methanol extracts were separated by using column chromatography. The isolated fractions were tested on tyrosinase inhibitory and DPPH scavenging activity. Fraction AHM 2, 6 and 7 exhibited good activities at %tyrosinase inhibition 37.50±1.85, 40.15±4.81 and 43.60±7.49, respectively and %DPPH radical scavenging activity were 31.95±1.36, 21.48±8.89 and 40.54±1.18, respectively. AHM 2, 6 and 7 were further fractionated by column chromatography and all fractions were tested for the activities. The result indicated that AHM 7-1 showed %tyrosinase inhibition at 29.58 ±3.57. AHM 6-4 showed %DPPH scavenging activity at 79.01±7.45. Chemical constituents of intested fractions were detected by TLC with spraying reagents, e.g. dragendorff, vanillin - sulfuric acid and ferric chloride. AHM 6-4 and AHM 7-1 illustrated positive result with vanillin - sulfuric acid. It can be implied that AHM 6-4 and AHM 7-1 contained phenolic compound.
   
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