Abstract

Title : Determination of Sennoside A in callus and hairy roots of genus Cassia
By : Jira Jongcharoenkamol Anyamanee Supamart
Degree : BACHELOR OF PHARMACY
Advisor : Thaweesak Juengwatanatrakul
Keywords : Senonside A, anti-sennoside A single-chain Fv, Cassia alata, Cassia angustifolia, Cassia fistula, Enzyme-linked immunosorbent assay, callus and hairy roots
   
Sennonside A, anthraquinone glycoside, is active component in the plants of the genus Cassia used as stimulant laxatives. This study aim to determination of sennoside A in callus and hairy roots of transgenic Cassia alata Linn. and Cassia angustifolia Vahl. by transfer an anti-sennoside A single-chain Fv (anti-SA ScFv) gene using Agrobacterium method. Sennoside A contents were determined by Enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody against sennoside A. The results show that the sennoside A content in transgenic callus of senna is higher than the control at 9.86 fold (10.17 ± 0.18 mg/g dry weight, p < 0.05). Transgenic callus and hairy roots of C. alata shows the sennoside A concentration higher than the control at 7.26 fold (3.07 ± 0.03 mg/g dry weight) and 78.23 fold (10.11 ± 1.37 mg/g dry weight), respectively.
   
Close this window