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Abstract |
Title |
: Determination of Sennoside A in callus and hairy roots of genus Cassia |
By |
: Jira Jongcharoenkamol
Anyamanee Supamart
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Degree |
: BACHELOR OF PHARMACY |
Advisor |
: Thaweesak Juengwatanatrakul |
Keywords |
: Senonside A, anti-sennoside A single-chain Fv, Cassia alata, Cassia angustifolia, Cassia fistula, Enzyme-linked immunosorbent assay, callus and hairy roots |
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Sennonside A, anthraquinone glycoside, is active component in the plants of the genus Cassia used as stimulant laxatives. This study aim to determination of sennoside A in callus and hairy roots of transgenic Cassia alata Linn. and Cassia angustifolia Vahl. by transfer an anti-sennoside A single-chain Fv (anti-SA ScFv) gene using Agrobacterium method. Sennoside A contents were determined by Enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody against sennoside A. The results show that the sennoside A content in transgenic callus of senna is higher than the control at 9.86 fold (10.17 ± 0.18 mg/g dry weight, p < 0.05). Transgenic callus and hairy roots of C. alata shows the sennoside A concentration higher than the control at 7.26 fold (3.07 ± 0.03 mg/g dry weight) and 78.23 fold (10.11 ± 1.37 mg/g dry weight), respectively.
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