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Abstract |
Title |
: Development of Test Kit for Quantitative Measuring of Total Hardness in Water |
By |
: Niroth Kiattikulvivat
Weerayuth Damprathik
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Degree |
: BACHELOR OF PHARMACY |
Advisor |
: Preecha Boonchoong
Zongporn Joungmunkong
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Keywords |
: Test kit, Water hardness, Eriochrome black T, Methylthymol blue |
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It is very important to measure the hardness of drinking water before consumption. Therefore the aim of this study was to develop a portable test for quantitative analysis of total hardness in drinking water. There were 10 test kits, which was developed in liquid formulation and consisted of disodium EDTA with various buffers (e.g., borate buffer, bicarbonate buffer, ammonia buffer or potassium chloride buffer) and indicators (e.g., eriochrome black T or methylthymol blue). The accuracy and stability after 2 month period of each kit was test by comparing with complexometric titration (standard method). This study revealed that after 5 days preparation each formulation showed the accuracy and precision in determination of hardness in water at the concentration ranged between 40-200 ppm. However, the test kits mixed with indicator were all unstable while the test kits containing disodium EDTA and each type of buffer were all stable. In addition, the kits consisted of methylthymol blue indicator and ammonia buffer were the most accurate and stable test. Of those formulations, Test kits consist of ammonia buffer and methylthymol blue indicator are the most accurate and stable formulations in quantitative measuring of hardness in drinking water at a range of 40-200 ppm.
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