Abstract

Title :

EFFECT OF ANG-KAP-NU (Barleria prionitis L.) AND OTHER 4 THAI HERBAL EXTRACTS ON RAT CYP3A4 ENZYME ACTIVITY IN-VITRO

By :

Mintra Sukuan

Thanawat Suriyon 


Degree : DOCTOR OF PHARMACY
Advisor :

Benjabhorn Sethabouppha 

Suttasinee Suwannakul  

Keywords :

Herb - Drug interaction, CYP3A4, Barleria prionitis L., Carissa carandas Linn.,                                Justicia gendarussa Burm.f., Limnocharis flava, Scaphium scaphigerum

   

Introduction: The use of herbal supplements has increased, and some herbal products may affect drug metabolism due to the interaction between herbs and medicines via cytochrome P450, major metabolizing enzyme. Objective: This research aimed to study the effect of Ang-kap-nu and other 4 Thai herbals crude extracts on function of rat liver CYP3A4 enzyme. Method: The metabolic test was carried out in vitro using testosterone as a specific CYP3A4 probe substrate. The amount of 6β-hydroxytestosterone obtained from metabolic reaction with or without herbal crude extract was determined using HPLC then covert to %inhibition. Result: The results revealed that the aqueous extracts of Ang-kap-nu and other 4 herbs inhibited CYP3A4 activity as Kra-duk-Kai-Dam (Justicia gendarussa Burm.f.) 79.41%, Ang-kap-nu (Barleria prionitis L.) 69.46%, Ma-muang-hao Ma-nao-ho (Carissa carandas Linn.) 58.06%,  Ta-la-pat-rue-si (Limnocharis flava) 51.15% and Luk-sam-rong (Scaphium scaphigerum) 39.81%. The ethanol extracts of Ang-kap-nu and other 4 herbs inhibited CYP3A4 activity as followings Ang-kap-nu (Barleria prionitis L.) 63.04%, Ma-muang-hao Ma-nao-ho (Carissa carandas Linn.) 61.41%, Kra-duk-Kai-Dam (Justicia gendarussa Burm.f.) 59.71, Ta-la-pat-rue-si (Limnocharis flava) 57.42% and  Luk-sam-rong (Scaphium scaphigerum) 49.16% The hexane extracts of Ang-kap-nu and other 4 herbs also exhibited the inhibitions of CYP3A4 function i.e. Luk-sam-rong (Scaphium scaphigerum) 56.55%, Ang-kap-nu (Barleria prionitis L.), Ta-la-pat-rue-si (Limnocharis flava) 47.40%, Kra-duk-Kai-Dam (Justicia gendarussa Burm.f.) 36.99% and Ma-muang-hao Ma-nao-ho (Carissa carandas Linn.) 33.39%. Conclusion: It could be concluded that all 5 tested Thai herbs inhibit CYP3A4 function in different degree.

   
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