Abstract

Title :

Angiogenesis activity of Dictyophora indusiata extracts

By :

Pornnatcha Salakaew

 Sumontakan phalaprom

Degree : DOCTOR OF PHARMACY
Advisor :

Autsana Puapermpoonsiri

Phijit Sritananuwat  

Keywords :

Dictyophora indusiata, CAM assay, Irritation, Angiogenesis

   

Introduction:  Dictyophora indusiata (Bamboo mushroom) has various reports in bioactivities such as antioxidants, antiinflammatory and immunomodulation that were increasingly being applied in cosmetic industry. However, there is no study about angiogenesis activity which is one of wound healing process. Wound is the important problems of skin problems, so we interest in angiogenesis activity to accelerate wound healing process. Objectives: This study examines irritation effect and angiogenesis activity of Dictyophora indusiata extracts on Chorioallantoic Membrane (CAM) assay. Methods: Powders of Chinese D. indusiata and Thai D. indusiata 50 g. were extracted by ethanol 400 ml for 24 hours, then extracted the precipitate by water for 6 hours. Prepare D. indusiata powders by using freeze dried method. Examine irritation effect and angiogenesis activity of extracts on CAM assay compared with 0.9% w/v NaCl (control for water part), DMSO (control for ethanol part). Observe change of vessels per time and images were recorded by digital camera with stereo zoom microscope. Results: No irritation effects on ethanol extract in DMSO solution 1/80 and water extract at concentrations 50 mg/ml, 20 mg/ml of Thai Ang Thong and Chinese species respectively. The results of the angiogenesis found that in the water extract at concentration 1 mg/ml (Thai Ang Thong species) and 0.1,1 mg/ml (Chinese species) have a tendency to increase %area of vascular density and vascular length density compared with control(NaCl 0.9%) while ethanol extract in DMSO at the concentration 2.5 mg/ml(Thai Ang Thong species) and 3.75 mg/ml (Chinese species)  have a tendency to increase %area of vascular density and vascular length density compared with control(DMSO1/80).  Conclusions: Irritation was not found in both water and ethanol extracts. Both water and ethanol extracts) have a tendency to increase angiogenesis activity.

   
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