Abstract

Development of modified Thai herbal compress using nanotechnology

Pattarapol Witchayapong

 Siripong Pochana

Sureewan Duangjit

“Micelle” “Curcumin” “Thai Herbal Compress” “Response surface method”

Introduction: Thai Herbal Compress (THC) is an alternative medicine. Clinical research supports that have a good efficacy in relieving back pain. But, the limitations of THC are inconvenient, unstable efficacy and the instability of herbs. Nowadays, a new type of THC was developed for convenience of use by extracting essential substances from the THC. However, the methods of extracting important substances from THC in green technique have not been studied yet. Objectives: The objective of this study was developed the micelle extraction method as an alternative green technique for the THC extraction. Moreover, the micelle extraction- and standard-method using the response surface method (RSM) were compared. Methods: Curcumin and/or compound D in Turmeric, Plai and THC were extracted in this study. Factors affecting the extraction, e.g., types of surfactant, concentration of surfactant and methods of extraction were demonstrated. The physicochemical properties (e.g., size, polydispersity index, zeta potential, concentration) of micelles were investigated. Moreover, the appropriate condition of micelle extraction was optimized by the response surface method. The ethanolic extraction was used as the standard method in this study. Results: The concentration of Curcumin was highly extracted with Triton X. The Triton X between 5 to 20 percentage and sonication time between 60 to 180 mins were the appropriate condition for the RSM. The size of micelle of Turmeric, Plai, and THC extracts was 16.4 to 4345 nm with polydispersity index 0.19 to 1. The zeta potential was between -0.6 to -12.5 mV. The concentration of curcumin extracted was 318.86 µg/mL and 64.33 µg/mL in turmeric and THC, respectively. The concentration of curcumin extracted by 95% ethanol in turmeric and THC was 235 µg/mL and 26.15 µg/mL. 5% and 9.10% of Triton X and sonication time 60 mins was the optimal condition for micelle extraction of turmeric and THC. Conclusion: