| |
Abstract |
| Title |
: Preminary screening test for the effect of Kotesor and 4 Thai herbal exract on rat CYP3A4 enzyme activity in-vitro. |
| By |
: Pantida Silaket
Patcharida Thitayaruk |
| Degree |
: BACHELOR OF PHARMACY |
| Advisor |
: Benjabhon Sethabouppha
Suttasinee Suwannakul
|
| Keywords |
: Herbal-drug interaction, Cytochrome P450, CYP3A4, Cuminu cyminum, Nigella sativa, Dracaena loureiri, Saussurea lappa, Angelica dahurica.
|
| |
|
| In the present, people are widely known herbal medicines for treatment of common illnesses either as a single drug or combination with other modern drugs. It is possible to cause herbal-drug interaction by affecting to Cytochrome P450 enzyme function. This research aimed to study the effect of Angelica dahurica and other 4 Thai herbal crude extracts on function of rat CYP3A4 enzyme. Metabolic tests were carried out in triplicate in vitro using Testosterone (TST) as a specific CYP3A4 probe substrate. The amount of 6β-hydroxytestosterone (6β-TST), major matabolite of TST, obtained from each matabolic reaction with or without herbal crude extracted (in alcohol, water and hexane)was determined using High Performance Liquid Chromatography(HPLC) technique then converted into % inhibition. Result revealed that the aqueous extracts of 5 herbs were affected CYP3A4 enzyme function in different degree of inhibition i.e. Tienkao(Cuminum cyminum)(10.56%), Tiendam(Nigella sativa)(8.79%), Jandang(Dracaena loureiri)(30.53%), Kotekradook(Saussurea lappa)(24.9%) and Kotesor (Angelica dahurica)(44.7%). The alchoholic extracts of 5 herbs inhibited CYP3A4 activity i.e. Tien-kwaw(Cuminum cyminum)(3.11%), Tiendam(Nigella sativa)(31.22%), Jandang(Dracaena loureiri)(39.56%), Kotekradook(Saussurea lappa)( 35.50%) and Kotesor (Angelica dahurica)(41.16%). The hexane extracts of 5 herbs also inhibited CYP3A4 activity i.e. Tien-kwaw(Cuminum cyminum)(24.07%), Tiendam(Nigella sativa)(23.37%), Jandang(Dracaena loureiri)(2.46%), Kotekradook(Saussurea lappa)(22.75%) and Kotesor (Angelica dahurica)(25.99%). It could be conclude that all 5 Thai herbs inhibit CYP3A4 function in different degree.
|
| |
|
| Close this window |