Abstract

Title : DETECTION OF β-LACTAMASE AND ANTIBIOTIC RESISTANT GENE IN MULTIPLE DRUG-RESISTANT Acinetobacter baumannii
By : SARAWUT SEMARAM TANYAPONG KUNAWATANAKUL SUPPADET SANGSANON
Degree : BACHELOR OF PHARMACY
Advisor : SIRIMA SUWANNAKUT
Keywords : Acinetobacter baumannii, β –lactamase, Antibiotic resistant gene
   
Acinetobacter baumannii is a gram negative bacterium that causes high incidence of drug resistant in the patients admitted to the hospitals, especially in an intensive care unit. The objectives of this study were to detect β –lactamases and antibiotic resistant genes in fifteen isolates of multiple drug-resistant Acinetobacter baumannii, isolated from Sappasitthiprasong Hospital. Productions of AmpC β-lactamase and carbapenemase were screened by AmpC disk test and Modified Hodge test, respectively. AmpC β-lactamases were produced by six (40%) isolates and 11 (73.33%) isolates were determined as potential carbapenemase producers. Detection of antibiotic-resistant genes by Polymerase Chain Reaction (PCR) technique showed that thirteen (86.67%) isolates were harbored blaOXA-23-like gene and blaIIMP-like gene was identified in 4 (26.67%) isolates.
   
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