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Abstract |
| Title |
: = PRODUCTION OF 9-HYDROXYCANTHIN-6-ONE FORM ROOT
CULTURE OF EURYCOMA LONGIFOLIA JACK AND EURYCOMA HAMANDIANA PIERRE.
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| By |
: PACHARAPONG CHOMTHONG
TAWIN THIANWAN,
NARATIP UDOMCHAWEE
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| Degree |
: BACHELOR OF PHARMACY |
| Advisor |
: THAWEESAK JUENGWATANATRAKUL
ZONGPORN JUENGMANKONG
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| Keywords |
: 9-HYDROXYCANTHIN-6-ONE, EURYCOMA LONGIFOLIA, EURYCOMA HAMANDIANA, ROOT CULTURE, HPLC
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| Root cultures of Eurycoma longifolia Jack (EL). and Eurycoma hamandiana Pierre (EH) was cultured in ½ WP with 0.4µg/ml NAA and 0.1% activated charcoal. Production of 9-hydroxycanthin-6-one in root cultures were determined after 3 days treatment of methyl jasmonate 100 µM and 200 µM. and performed by HLPC. The concentrations of 9-hydroxycanthin-6-one of the root culture of EL and EH, control groups, are 2.44 µg/100 mg, and 0.97 µg/100 mg, respectively. Methyl jasmonate enhanced the intracellular 9-hydroxycanthin-6-one content of the EL root culture by 2.97-fold at 200 µM of methyl jasmonate and EH root culture by 2.71-fold at 100 µM of methyl jasmonate. |
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