Abstract

Title : CHARACTERIZATION AND IDENTIFICATION OF LACTOBACILLUS FOR POTENTIAL PROBIOTIC
By : KANDANAI JANSONG CHANTHIMA PHIANTHAM PARAMEE POOKHAOW
Degree : BACHELOR OF PHARMACY
Advisor : SIRIMA SUWANNAKUT CHUTINUN PRASITPURIPRECHA, Ph.D.
Keywords : PROBIOTIC, LACTOBACILLUS, ANTI-HELICOBACTER PYLORI, IDENTIFICATION, ANTIBIOTIC SUSCEPTIBILITY
   
The objectives of this study were to characterize and identify five potentially probiotic Lactobacillus isolates, obtained from previous study. Detection of anti-Helicobacter pylori activity by agar diffusion showed that all five lactobacillus isolates exhibited anti-H. pylori activity. Determination of factors affecting the anti-Helicobacter pylori activities by agar diffusion including enzymes and solvents found that an anti-Helicobacter pylori activities of five isolates were decreased by Proteinase K while lysozyme decreased anti-H.pylori activity of the isolate, P70. All solvents tested including chloroform, acetonitrile, ethanol and methanol decreased only the anti-H. pylori activity of the isolate P29C3. The determination of antibiotic susceptibility to six antibiotics including ampicillin, tetracycline, clarithromycin, norfloxacin, co-trimoxazole, and gentamicin found that all five isolates were resistant to co-trimoxazole and were sensitive to ampicillin and clarithromycin. Preliminary identification by Gram’ stain, catalase test, and biochemical test using API 50 CHL test kit allowed the identification of three isolates as L. paracasei ssp. paracasei 1, one as Lactococcus lactis ssp. lactis 1, and one as L. paracasei ssp. paracasei 2.
   
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