|The purposes of this study were to invsetigate the antimicrobial activity and hair growth stimulation of 2 plant extracts and 4 volatile oils. The investigated plant extracts and volatile oils were Pueraria mirifica, soybean, lemongrass oil, citronella oil, basil oil and peppermint oil. Antimicrobial activity was investigated using agar disc diffusion and agar dilution assay. The hair growth promotion was investigated using organ culture and dermal papilla cells culture. Results showed that the volatile oil had antimicrobial activity. Basil oil, lemongrass oil, citronella oil and peppermint oil had the activity against organisms with the minimal inhibitory concentration (MIC) to S.aureus of 12.50, 3.13, 12.50, 6.25 mg/ml and M.furfur of 6.25, 0.78, 12.50, 6.25 mg/ml, respectively. While the plant extract had no antimicrobial activity. The growth of hair follicle cultured in vitro can be promoted by low concentration (0.0001 µg/ml) of all volatile oil encapsulated noisome. Only basil oil encapsulated niosome at all concentration had no effect on hair growth. And the dermal papilla cell (DPCs) culture, P. mirifica extract at 500 µg/ml and 0.005 µg/ml stimulated the proliferation of DPCs on day 1 and day 5 of the experiment. The niosome of P. mirifica (0.001 µg/ml) soybean (100 µg/ml) lemongrass oil (0.1-10 µg/ml) citronella oil and peppermint oil (0.01-100 µg/ml) stimulated the proliferation of DPCs on day 5 of the experiment. This can be concluded that lemongrass oil showed strongest antimicrobial activity and the niosome of P. mirifica, soybean, lemongrass oil, citronella oil and peppermint oil exhibited better hair growth stimulation than the unencapsulated plant extract and volatile oil.